The Rabbit Anti-Pyruvate Dehydrogenase E1-alpha Subunit Antibody from MyBioSource.com is a Rabbit Polyclonal antibody to PDHA1, and pyruvate dehydrogenase E1 subunit alpha 1. This antibody recognizes Human antigen. The Rabbit Anti-Pyruvate Dehydrogenase E1-alpha Subunit Antibody has been validated for the following applications: Immunocytochemistry, Immunofluorescence, and Western Blot.
Description
The pyruvate dehydrogenase complex (PDC) is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293 which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites